Gong Yoo first met Im Soo Jung when they appeared togetherg in the television series School4 in The series marked the debut of both actors. They were paired together in other works several times after that too. Moreover, they belong to the same company. Gong Yoo was rumored to . Jan 08, · New year yet same old dating rumor to start things off on the second weekend. K-stars Gong Yoo and Jung Yumi were swept up in a wedding rumor today and both stars mutual agency has released an official response denying the rumor. An anonymous post claiming by an employee of Shilla Hotel saying that Gong Yoo reserved the famed venue for an upcoming wedding and the bride was to .
In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated cytotoxic T cell CTL peptide epitopes, alone or in combination with other tumor-associated peptides that serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses. The present invention relates to peptide sequences and their variants derived from HLA class I and class II molecules of human tumor cells that can be used in vaccine compositions for eliciting anti-tumor immune responses.
Claims are shown in the official language in which they were submitted. The peptide according to claim 1 or 2, wherein the amino acid sequence thereof comprises a continuous stretch of amino acids according the group of SEQ ID No. The peptide according to any of claims 1 to 3, wherein said peptide or variant thereof has an overall length of from 8 to , preferably from 8 to 30, and more preferred from 8 to 16 amino acids, and most preferred wherein the peptide consists or consists essentially of an amino acid sequence according to SEQ ID No.
The peptide according to any of Claims 1 to 5, wherein said peptide is part of a fusion protein, in particular comprising N-terminal amino acids of the HLA-DR antigen- associated invariant chain Ii.
A nucleic acid, encoding a peptide according to any one of claims 1 to 6, optionally linked to a heterologous promoter sequence. An expression vector capable of expressing the nucleic acid according to claim 7.
The peptide according to any one of claims 1 to 6, the nucleic acid according to claim 7 or the expression vector according to claim 8 for use in medicine. A host cell comprising the nucleic acid according to claim 7 or the expression vector according to claim 8, wherein said host cell preferably is an antigen presenting cell or A method for producing the peptide according to any one of claims 1 to 6, the method comprising culturing the host cell according to claim 10 that expresses the nucleic acid according to claim 7 or the expression vector according to claim 8, and isolating the peptide from the host cell or its culture medium.
An in vitro method for producing activated cytotoxic T lymphocytes CTL , the method comprising contacting in vitro CTL with antigen loaded human class I or II MHC molecules expressed on the surface of a suitable antigen-presenting cell or an artificial construct mimicking an antigen-presenting cell for a period of time sufficient to activate said CTL in an antigen specific manner, wherein said antigen is a peptide according to any one of claims 1 to 9.
An activated cytotoxic T lymphocyte CTL , produced by the method according to claim 12, that selectively recognises a cell which aberrantly expresses a polypeptide comprising an amino acid sequence given in any one of claims 1 to 5.
A method for killing target cells in a patient which target cells aberrantly express a polypeptide comprising an amino acid sequence given in any one of claims 1 to 5, the method comprising administering to the patient an effective number of cytotoxic T lymphocytes CTL as defined in claim An antibody that specifically recognizes the peptide according to any of claims 1 to 5, preferably the peptide according to any of claims 1 to 5 that is bound to an MHC molecule.
Use of a peptide according to any one of claims 1 to 7, the nucleic acid according to claim 7, the expression vector according to claim 8, the cell according to claim 10, the activated cytotoxic T lymphocyte according to claim 13 ot the antibody according to claim 15 for the treatment of cancer or in the manufacture of a medicament against cancer, wherein said medicament preferably is a vaccine.
The use according to claim 16, wherein said cancer is selected from astrocytoma, pilocytic astrocytoma, dysembryoplastic neuroepithelial tumor, oligodendrogliomas, ependymoma, glioblastoma multiforme, mixed gliomas, oligoastrocytomas, medulloblastoma, retinoblastoma, neuroblastoma, germinoma, teratoma, gangliogliomas, gangliocytoma, central gangliocytoma, primitive neuroectodermal tumors PNET, e.
A kit comprising: The kit according to claim 18, further comprising one or more of iii a buffer, iv a diluent, v a filter, vi a needle, or v a syringe.
A method for producing a personalized anti-cancer vaccine for an individual patient, a identifying tumor-associated peptides TUMAPs presented by a tumor sample from b comparing the peptides as identified in a with a warehouse of peptides that have been prescreened for immunogenicity and overpresentation in tumors as compared to c selecting at least one peptide from the warehouse that correlates with a tumor- associated peptide identified in the patient; and d manufacturing the personalized vaccine based on step c.
The method according to claim 21 or 22, wherein the sequences of MHC ligands is identified by eluting bound peptides from MHC molecules isolated from the tumor sample, and sequencing the eluted ligands. The method according to any of claims 21 to 23, wherein the normal tissue corresponding to the tissue type of the tumor sample is obtained from the patient. The method according to any of claims 21 to 24, wherein the peptides included in the warehouse are identified according to: HLA ligands from the malignant material are identified by mass spectrometry; b.
Genome-wide messenger ribonucleic acid mRNA expression analysis by microarrays is used to identify genes over-expressed in the malignant tissue GBM compared with a range of normal organs and tissues; c.
The identified HLA ligands are compared to gene expression data; d. Peptides encoded by selectively expressed or over-expressed genes as detected in step e. To assess whether an induction of in vivo T-cell responses by the selected peptides is feasible, in vitro immunogenicity assays will performed using human T cells from healthy donors or the patient.
The method method according to any of claims 21 to 27, further comprising d identifying at least one mutation that is unique to the tumor sample relative to normal corresponding tissue from the individual patient, and selecting a peptide for inclusion in the vaccine that correlates with the mutation.
The method of claim 28, wherein said at least one mutation is identified by whole.
Eun Hye played as a tomboy in 1st Shop of Coffee. But he did not wanted people to think like that and said. But agency clear it up. Gong Yoo made a promise that he will announce first to his fans when he finally get married. Gong Yoo admitted in the interview that he need to get hitched before at the age of 40 and furthermore have a youngster. He said that he will get a kick out of the chance to have a lady that he cherishes and adores him as well, moreover he conteinued to state that marriage life will be hard on occasion however he need to feel everything regardless of how hard it is.
Gong also consider how it resembles to see his child who takes after him gazes at him. He said: I wonder how its like to see my baby who resembles me. These results was updated on January 19, From these, the movie was able to clinch ten awards in different categories. While he has preferred not to talk openly about his private life, we know that as of the second half of , he is still without a wife, and he has never been married before.
That stated, he has been linked to a number of women in the past. But it was until that they were rumored to be dating. By , there were reports that they were even secretly married, but as we now know, it is not true. The rumor about an affair between the duo was so serious that their agencies had to come out and point out that there was no truth in the claim. Even though the rumor went wild again, the agencies representing the Silenced and Train to Busan actors denied the report, threatening legal action.
Apart from these women, it is not known is the Gong has been in any relationship in the past or if he is in any at the moment. She even said later that the kiss scene was the best in her life. Their romantic relationship was revealed by some pictures about their kissing and dating which were leaked on the internet.
After that, the two denied the report officially through their companies because the dating rumors spread so widely. His Ideal Type of Woman Gong Yoo has finally opened up and told the media what kind of a person would be considered his ideal type. Gong Yoo said in the TV show that his ideal types seemed different from what he had when he was younger, responding to a question about what type of woman he likes. The actor also mentioned that he would like some one who is a bit of a tomboy. He mentioned that innocent women are to his opinion somewhat boring and that he is more attracted to a tomboys.
Gong Yoo even said that actress Yoon Eun Hye, who co-starred with him in Coffee Prince, is someone who fits the category of his ideal type.